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LSY-20105 Beta-lactams, Tetracyclines, Melamine and Cephalexin Combo rapid test

Shenzhen Lvshiyuan Biotechnology Co.,Ltd
Shenzhen Lvshiyuan Biotechnology Co.,Ltd
City: Shenzhen
Province/State: Guangdong
Country/Region: China
Tel: +86-755-28438788-8030
Contact Person:Mrs Bella Zou

LSY-20105 Beta-lactams, Tetracyclines, Melamine and Cephalexin Combo rapid test

Brand Name : Green Spring
Model Number : LSY-20105
Certification : ISO9001&ISO14001&OHSMS18001
Place of Origin : China
MOQ : 1 box
Price : FOBShenzhen$336
Payment Terms : T/T, Western Union, MoneyGram
Supply Ability : 1000 kits/day
Delivery Time : 3 working days
Packaging Details : By cardboard box
Specifications : 96 strips/kit
Testing time : 5min-5min
Shelf life : 12 months
Storage : Store at 2~8℃
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Beta-lactams, Tetracyclines, Melamine and Cephalexin Combo rapid test strip

Catalogue No.: LSY-20105

1. Brief

The Beta-lactams, Tetracyclines, Melamine and Cephalexin Combo rapid test strip, based on Colloidal gold immunochromatography assay method, is used for testing Beta-lactams, Tetracyclines, Melamine and Cephalexin residues in milk and goat milk quickly.


  • Specifications

(1) 8 microwells and 8 dipsticks in one bottle 12 bottles / kit.

(2) Instruction 1 piece


3. Principle

The Beta-lactams, Tetracyclines, Melamine and Cephalexin Combo rapid test strip is based on competitive inhibition immuno-chromatographic principle. When the antibiotics residue in sample is higher than or equal to the detection limit, it will combine with labeled antibody, then inhibit the combination between colloidal gold antibody coated antigen on T line, making T line lighter than C line or T line invisible or T line has same color with C line. Otherwise, T line is darker than C line. No matter whether there is antibiotics residue in sample, C line is visible.


4. Detection limit (See Tablet 1)


5. Operation procedures

1) Read the manual carefully before testing, return test strips and milk sample into room temperature before use.

2) Take bottles needed from the kit package, take out required microwells, making proper marks, Please use it in 1 hour. Seal the cap of the bottles, avoid moisture.

3) Use Micro-pipette to take 200ul of the samples into the microwells, then repeatedly absorb for 5~10 times, mix the sample with the reagent in the microwells completely until no solid judged by eyes (this is a very important step).

4) Incubate at (40±2℃) for first step, timing for 5min.

5) Then insert the strip into micro-well, put the end with MAX down into the liquid, Incubate at (40±2℃) for second step, timing for 5min.

6) Take out the strip and read the result as the picture, it is invalid to read result in other times.


6. Test Result Interpretation

Color depth comparison of T line and C lineResult judgmentResult analysis
T line > C lineNegativeantibiotics residue is lower than detection limit in sample
T line = C lineWeak positiveantibiotics residue is equal to detection limit in sample
T line < C line or T line is invisiblePositiveantibiotics residue is higher than detection limit in sample
C line is invisibleInvalidThe test dipstick is invalid, test again

7. Results picture show


8. Precautions

1) This product is suitable for pure milk of cattle and sheep and raw milk.

2) Test dipsticks and micro-wells are disposable,do not use test dipsticks out of date.

3) Please seal the bottle after taking out required test dipsticks. If can not use all 8 microwells at once, cup the rest and put it back to bottle and sealed.

4) Do not touch the white membrane surface in the middle of test dipsticks, avoid sunlight and fan blowing directly.

5) Store milk sample in cold, no more than 3 days.

6) Milk samples should be fully liquid without any agglomeration, sour and precipitation. Stir milk sample to even before test.

7) For milk sample with high fat content, chromatography is slow, recommending extension the second step for another 1 min.

8) Use the test dipsticks testing again for positive results.

9. Specificity

This product has no cross-reaction with Quinolones, Sulfonamides and Chloramphenicol etc.

10. Storage and expiry date

Storage: Store at 2-8 ℃ in sealed, dry, and dark place, no frozen.

Expiry date: 12 months; date of production is on box.


Tablet 1

β-lactams

Detection limit

(ppb)

β-lactams

Detection limit

(ppb)

Tetracyclines

Detection limit

(ppb)

Detection limit

(ppb)

Penicillin G2~4Cefquinome8~15Tetracycline10-15Cephalexin10-15
Amoxicillin2~3Cefalotin30~40Oxytetracycline10-15Melamine10-15
Ampicillin2~3Cefazolin40~50Chlortetracycline10-15
Oxacillin5~7Cephalonium8~10Doxycycline15-20
Cloxacillin4~6Cefacetrile20~30
Piperacillin6~8Cefoperazone3~5
Nafcillin15~25Cephapirin8~10
Dicloxacillin6~8Ceftiofur60~100


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